3-Aminotriazole
From OpenWetWare
(Difference between revisions)
(adapted to template) |
|||
| Line 1: | Line 1: | ||
| - | 3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies. | + | ==Purpose== |
| + | *3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies. | ||
| - | + | ==Procurement== | |
| + | You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma]. | ||
| - | + | ==Use== | |
| + | *Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM. | ||
| - | References | + | ==Safety== |
| + | *Possible carcinogen. Use gloves, work in a hood. | ||
| + | |||
| + | ==References== | ||
[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information] | [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information] | ||
[[Category:Material]] [[Category:Chemical]] | [[Category:Material]] [[Category:Chemical]] | ||
Revision as of 04:53, 7 March 2008
Contents |
Purpose
- 3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.
Procurement
You can buy 3-amino-1,2,4-triazole for example at Sigma.
Use
- Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.
Safety
- Possible carcinogen. Use gloves, work in a hood.
