From OpenWetWare

Revision as of 17:07, 31 July 2006 by Josh K. Michener (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.

Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.

Possible carcinogen. Use gloves, work in a hood.

References: Sigma product information

Personal tools