7-AAD: Difference between revisions
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(New page: 7-aminoactinomycin-D * 546/647 * Nucleic acid dye * Stains '''dead cells''' * "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluores...) |
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7-aminoactinomycin-D | 7-aminoactinomycin-D | ||
==Purpose== | |||
* Staining '''dead cells''' | |||
==Procurement== | |||
* Most standard companies | |||
** [http://www.bdbiosciences.com/ptProduct.jsp?prodId=12856 BD Biosciences] | |||
** [http://www.ebioscience.com/ebioscience/specs/antibody_00/00-6993.htm eBioscience] | |||
==Use== | |||
* 546/647 | * 546/647 | ||
* Nucleic acid dye | * Nucleic acid dye | ||
* | * Often used in conjunction with [[Annexin-V]] when assaying for apoptotic cells | ||
* "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter). | * "The '''advantage of 7-AAD''' over PI is the ability to be used '''in conjunction with''' phycoerythrin ('''PE''')- '''and''' fluorescein isothiocyanate ('''FITC''')-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter). | ||
==Safety== | |||
* No significant safety considerations ([http://www.bdbiosciences.com/external_files/pm/doc/msds/live/web_enabled/559925-MSDS.01.pdf BD Biosciences MSDS Sheet]) | |||
==References== | |||
* MC O’Brien, et al. (1995) Cytometry 19:243 | |||
* I Schmid et al. (1992) Cytometry 13:204 | |||
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Latest revision as of 06:30, 5 May 2009
7-aminoactinomycin-D
Purpose
- Staining dead cells
Procurement
- Most standard companies
Use
- 546/647
- Nucleic acid dye
- Often used in conjunction with Annexin-V when assaying for apoptotic cells
- "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Safety
- No significant safety considerations (BD Biosciences MSDS Sheet)
References
- MC O’Brien, et al. (1995) Cytometry 19:243
- I Schmid et al. (1992) Cytometry 13:204
