7-AAD
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(Difference between revisions)
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7-aminoactinomycin-D | 7-aminoactinomycin-D | ||
| + | ==Purpose== | ||
| + | * Staining '''dead cells''' | ||
| + | |||
| + | ==Procurement== | ||
| + | * Most standard companies | ||
| + | ** [http://www.bdbiosciences.com/ptProduct.jsp?prodId=12856 BD Biosciences] | ||
| + | ** [http://www.ebioscience.com/ebioscience/specs/antibody_00/00-6993.htm eBioscience] | ||
| + | |||
| + | ==Use== | ||
* 546/647 | * 546/647 | ||
* Nucleic acid dye | * Nucleic acid dye | ||
| - | * | + | * "The '''advantage of 7-AAD''' over PI is the ability to be used '''in conjunction with''' phycoerythrin ('''PE''')- '''and''' fluorescein isothiocyanate ('''FITC''')-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter). |
| - | + | ||
| + | ==Safety== | ||
| + | * No significant safety considerations ([http://www.bdbiosciences.com/external_files/pm/doc/msds/live/web_enabled/559925-MSDS.01.pdf BD Biosciences MSDS Sheet]) | ||
| + | |||
| + | ==References== | ||
| + | * MC O’Brien, et al. (1995) Cytometry 19:243 | ||
| + | * I Schmid et al. (1992) Cytometry 13:204 | ||
| + | |||
| + | <!-- ==Contact== | ||
| + | |||
| + | Add any other categories that may be relevant to your page. We use categories like tags. | ||
| + | --> | ||
| + | [[Category:Material]] | ||
Revision as of 03:47, 8 July 2008
7-aminoactinomycin-D
Contents |
Purpose
- Staining dead cells
Procurement
- Most standard companies
Use
- 546/647
- Nucleic acid dye
- "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Safety
- No significant safety considerations (BD Biosciences MSDS Sheet)
References
- MC O’Brien, et al. (1995) Cytometry 19:243
- I Schmid et al. (1992) Cytometry 13:204
