7-AAD

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7-aminoactinomycin-D

Contents

Purpose

  • Staining dead cells

Procurement

Use

  • 546/647
  • Nucleic acid dye
  • Often used in conjunction with Annexin-V when assaying for apoptotic cells
  • "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Safety

References

  • MC O’Brien, et al. (1995) Cytometry 19:243
  • I Schmid et al. (1992) Cytometry 13:204
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