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| '''Post discussion, questions, or comments about the course material here.''' | | '''Post discussion, questions, or comments about the course material here.''' |
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| ==Week 1: Introduction== | | ==[[7.342: Week 1 Questions|Week 1: Introduction]]== |
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| ==Week 2: Chromatin Functions to Define Cell State== | | ==[[7.342: Week 2 Questions|Week 2: Chromatin functions to define cell state]]== |
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| . | | ==[[7.342: Week 3 Questions|Week 3: Chromatin structure and discovery of chromatin modifying enzymes]]== |
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| Questions about the first paper: | | ==[[7.342: Week 4 Questions|Week 4: Methylation and the histone code]]== |
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| I don't really understand why ATV genes are sensitive to digestion. The paper says the globin genes are active, and therefore more have an altered subunit structure, which is more susceptible to pancreatic DNAse digestion. I don't understand how this fits with less active RNA tumour virus genes also being digested.
| | ==[[7.342: Week 5 Questions|Week 5: Heritable gene expression]]== |
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| | ==[[7.342: Week 6 Questions|Week 6: Pluripotency regulators and stem cell differentiation]]== |
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| Question about the second paper:
| | ==[[7.342: Week 7 Questions|Week 7: Regulation of Early Development by Polycomb Proteins]]== |
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| In figure 4C, the NPCs show a very clear line on the gel electrophoresis, which is not seen on the ESCs. This is present both before and after digestion with micrococcal nuclease. What does this line represent? It suggests some undigestible element of the nucleus, present in NPCs, and not in ESCs. I'd investigate this further.
| | ==[[7.342: Week 8 Questions|Week 8: Master Regulators of Differentiation: The Story of MyoD]]== |
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| -Manpreet
| | ==[[7.342: Week 9 Questions|Week 9: Transdifferentiation, Dedifferentiation, and the Adoption of Alternate Cell Fates]]== |
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| | ==[[7.342: Week 10 Questions|Week 10: The RNAi machinery and chromatin modifications]]== |
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| 1) For the Weintraub paper, I was wondering if today we have images of the
| | ==[[7.342: Week 11 Questions|Week 11: Chromatin Misregulation in Cancer]]== |
| histone conformations they were speculating about at that time.
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| 2) For the Meshorer one, I was curious about the leukemia inhibitory factor
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| (LIF) and why the cell differentiates when you deplete it.
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| -Amber
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| First paper:
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| What exactly are they doing with the acid precipitations/what do the acid-soluble fractions contain? (e.g. in pg. 849 middle column, pg. 850 middle column, figure 2 legend, pg. 853 middle column).
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| Having a hard time fully understanding the x-axis of their graphs... Cot is [DNA]*(time digested)?? Concentration of which DNA? I don't know how to predict what the curves should look like with those units.
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| Second paper:
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| What is FISH?
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| The HirA-/- data are the opposite of what I would expect. Since lack of HirA makes it harder to assemble complete nucleosomes ("reduced incorporation of core histones H3 and H3.3"), then it would seem that HirA-/- cells would be less able to form heterochromatin, and therefore prefer to stay more ESC-like rather than differentiating quickly. The authors' argument is that since H3 and H3.3 cannot be incorporated as well, they are more of them floating around; but in the end wouldn't you still need HirA function to use those extra H3/H3.3s?
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| -Kathy
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| Questions relating to Paper 1 (Chromosomal subunits in active genes have an
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| altered conformation)
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| -It says that mature adult RBCs that don't synthesize RNA are also sensitive to
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| the nuclease - does this suggest that the original structure is not reinstated?
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| Could this be due to the lack of hyperdynamic chromatin proteins shown in paper
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| 2? Does it also suggest that it isn't the structure of DNA that is manipulated
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| to silence the gene? In which case what is used?
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| - Why does staph nuclease not normally show preferential digestion - is it
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| because even with the more open conformation of active genes the enzyme is
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| still to bulky(?) to access them?
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| - A possible control to check that the preferential digestion is due to
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| the structural conformation would be to digest the histones with a protease,
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| then subsequently add the nuclease - would expect 100% digestion?
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| Questions relating to Paper 2 (Hyperdynamic etc etc) | |
| - Possible follow up questions - What are the interactions of the hyperdynamic
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| chromatin proteins; what is the signal that instigates their actions in the
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| remodeling process? Is there anything that could reinstate the hyperdynamic
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| nature and would this cause the cell to revert back to pluripotency?
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| -Holly
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| For "Chromosomal subunits in active genes have an altered conformation", I'm
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| confused by the passage on page 849, in the middle column, where it describes
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| the DNA being 'nibbled'. If the DNA is being digested (and presumably
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| differently in different cells, since different cells have different active
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| genes), doesn't that mean that then each type of cell has a different set of
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| DNA? I thought all the cells in an organism had the same set of DNA... Do
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| they just start off with the same set, and then they're modified?
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| For "Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem
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| cells", I was confused by the discussion in the summary about chromatin binding
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| proteins. Are these proteins that simply bind to the chromatin, or are they
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| proteins that bind to multiple, pieces of chromatin and thus bind the chromatin
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| together, affecting the shape?
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| -Cathlene
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| ==Week 3: Chromatin structure and discovery of chromatin modifying enzymes==
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