840:153g/2008: Difference between revisions

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==Course overview==
==Course overview==
This page is the homepage of the course titled "840:153g".
This page is the homepage of the course titled "840:153g". Current version: Fall 2008!


We have 16 students in class which will form groups of two throughout the semester. Each group will clone their own gene of interest into E. coli and verify that they cloned a functional gene. Students have to select a gene and identify a suitable source for cloning it. They need to develop a strategy for cloning, including a procedure for verification that they actually cloned the full coding region of the gene. Each group of students will develop a website at OWW where they describe their project. It is required that student write a DAILY lab notebook during/after each lab session. This online notebook must contain all procedures carried out during the session, description of results, and discussion of the next steps. This notebook will be graded based on completeness, accuracy, and content. It will substitute for a paper lab notebook.
We have 16 students in class which will form groups of two throughout the semester. Each group will clone their own gene of interest into E. coli and verify that they cloned a functional gene. Students have to select a gene and identify a suitable source for cloning it. They need to develop a strategy for cloning, including a procedure for verification that they actually cloned the full coding region of the gene. Each group of students will develop a website at OWW where they describe their project. It is required that student write a DAILY lab notebook during/after each lab session. This online notebook must contain all procedures carried out during the session, description of results, and discussion of the next steps. This notebook will be graded based on completeness, accuracy, and content. It will substitute for a paper lab notebook.

Revision as of 19:53, 19 April 2008

840:153g: Recombinant DNA Te(a)chniques

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Course overview

This page is the homepage of the course titled "840:153g". Current version: Fall 2008!

We have 16 students in class which will form groups of two throughout the semester. Each group will clone their own gene of interest into E. coli and verify that they cloned a functional gene. Students have to select a gene and identify a suitable source for cloning it. They need to develop a strategy for cloning, including a procedure for verification that they actually cloned the full coding region of the gene. Each group of students will develop a website at OWW where they describe their project. It is required that student write a DAILY lab notebook during/after each lab session. This online notebook must contain all procedures carried out during the session, description of results, and discussion of the next steps. This notebook will be graded based on completeness, accuracy, and content. It will substitute for a paper lab notebook.

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17 April 2024

     15:34  BioMicroCenter:Element Sequencing‎‎ 3 changes history +295 [Challee‎ (3×)]
     
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16 April 2024

N    19:59  Nanoimprint Lithography (NIL) - Carter Paul‎‎ 10 changes history +7,205 [CarterPaul‎ (10×)]
     
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18:42 (cur | prev) +85 CarterPaul talk contribs (Created page with "{{Template:CHEM-ENG590E}} =Motivation= =Introduction to NIL= =Thermal NIL Process=")
     19:40 Upload log CarterPaul talk contribs uploaded File:NIL1.png
N    18:40  3D Cell Culture - McLean Taggart, Emma Villares, Maximillian Marek, Scott LeBlanc, Adam Lyons and Jacob Belden diffhist +24,060 CarterPaul talk contribs (Created page with "{{Template:CHEM-ENG590E}} ==Introduction== While most microfluidic devices incorporate a 2D cell culture design, in which a single layer of cells is grown on the bottom of a device, these systems suffer from poor <i>in vivo</i> mimicry, as, in the human body, most cells grow in all directions.<sup>https://doi.org/10.5114/aoms.2016.63743 1</sup> To address this limitation, 3D cell culture devices have been developed - in w...")
     18:38  CHEM-ENG590E:Wiki Textbook‎‎ 2 changes history +63 [CarterPaul‎ (2×)]
     
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     18:36  3D Cell Culture - McLean Taggart, Emma Villares, Maximillian Marek, Scott LeBlanc, and Adam Lyons diffhist +5,343 CarterPaul talk contribs (Added a Technique and applications section)
     10:20  Yarn Microfluidics - Roger Dirth‎‎ 12 changes history +442 [Rcostello‎ (12×)]
     
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     08:18  3D Printed Microfluidic Robots - Helen Hua‎‎ 2 changes history +6 [Michele Caggioni‎ (2×)]
     
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15 April 2024

     23:43  User:Yanbin Huang‎‎ 2 changes history +170 [Yanbin Huang‎ (2×)]
     
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     22:11  The paper that launched microfluidics - Xi Ning‎‎ 11 changes history +4,793 [Xning098‎ (11×)]
     
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     21:45  (Upload log) [Xning098‎ (2×)]
     
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21:30 Xning098 talk contribs uploaded File:Figure 3 Set-up3.png