840:153g:Projects: Difference between revisions

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==2012 (Fall) Projects ==


We have 16 students in class which will form groups of two throughout the semester. Each group will clone their own gene of interest into E. coli and verify that they cloned a functional gene. Students have to select a gene and identify a suitable source for cloning it. They need to develop a strategy for cloning, including a procedure for verification that they actually cloned the full coding region of the gene. Each group of students will develop a website at OWW where they describe their project. It is required that student write a DAILY lab notebook during/after each lab session. This online notebook must contain all procedures carried out during the session, description of results, and discussion of the next steps. This notebook will be graded based on completeness, accuracy, and content. It will substitute for a paper lab notebook.
[[/project27|Project 27: Expression of methane monooxygenase in ''E. coli'' for biodegradation of methane and trichloroethylene. By Austin Jones and Jace Dolphin]]
 
[[/project26|Project 26: The Cloning of Atrolysin A from Crotalus atrox and "Stone Cold" and Kayla Ohrt & AJ Goos]]
 
[[/project25|Project 25: Cloning of MreB from Caulobacter ''crescentus'' into E. ''coli''. "Tango-Alpha" (Colby Swanson & Bess Lippmann)]]
 
[[/project24|Project 24: Over expression of Acetyl- CoA carboxylase (ACC) sub-unit accC in E.coli to enhance fatty acid accumulation for Bio-fuel production”."Fuel-it-up" (Sanju Timilsina and Parul Sirohi)]]
 
[[/project23|Project 23: Cloning of the Outer Membrane f-type Cytochrome (OmcF) gene for the production of Bioelectricity by Valerie Wisco and Casey Durnan]]
 
[[/project22|Project 22: Production of prodigiosin in ''E. Coli'' by Team Jacob (Stephanie Vondrak, Brian Hovey)]]
 
[[/project21|Project 21: Cloning of PMT1149 antifreeze gene into ''E. Coli'' "Frozen Assets" (Brandon Ramirez and Serge Marraback)]]
 
==2011 (Fall) Projects ==
 
 
[[/project20|Project 20: Cloning and expression of neutral protease gene from B. Stearothermophilus / (TIC)]]
 
[[/project19|Project 19: Cloning tps1 gene in Citrus Sinensis/ Orange you glad we didn't say Banana? (Beth, Cassidy, OJ)]]
 
[[/project18|Project 18: Cloning the gene of Acenitobacter calcoaceticus strain ADP1 to observe its expression in E.coli / GAS]]
 
[[/project17|Project 17: Cloning the Progesterone 5-beta Reductase Gene from Selaginella moellendorffii (Charlie, Sheree, Amanda)]]
 
[[/project15|Project 15: G.tigrina Hox Gene Dthox C Insertion Into Prokaryote E.coli / Team UNIamCloning (Alex, Courtney)]]
 
[[/project14|Project 14: Cloning of the Orcinol O-methyltransferase (OOMT2) Gene in Roses (Megan, Kim)]]
 
==2010 (Fall) Projects ==
[[/project13|Project 13: Detecting Estrogen in Water with Florescence "Team Detectors"]]
 
[[/project12|Project 12: Oscillating fluorescence in E. coli and "Team BAs"]]
 
[[/project11|Project 11: E. coli fluorescing based on cold temp sensor and "E cool I"]]
 
[[/project10|Project 10: Remediation of Phenol Resin using White Rot LiP transferred into E. Coli and "team Fun Guys"]]
 
[[/project9|Project 9: Tetrodotoxin Production in ''E. coli'' Using Pufferfish ''Flp'' Genes.  "Team Petrificus Totalus"]]
 
==2009 (Fall) Projects ==
[[/project8|Project 8: Human Factor X: The NeXt generation of clotting]]
 
[[/project7|Project 7: E. coli Tic Tacs]]
 
[[/project6|Project 6: Strawberry Shortcake: An Exploration of the FaQR Gene]]
 
==2009 (Spring) Projects ==
[[/project5|Project 5: NarL promotor manipulation "g(NarL)y prom(-O)ters"]]
 
[[/project4|Project 4: Anti-Fungal Bacteria "MoldBusters"]]
 
[[/project3|Project 3: Tear Inducing Bacteria by "It's my E. coli and I'll cry if I want to"]]
 
==2008 (Fall) Projects==
[[/project2|Project 2: The sweet smell of ...''E.coli''? ]]
 
[[/project1|Project 1: Our Field of Dreams by "Emblazon"]]
 
 
 
== Templates (please don't delete) ==
 
[[/project_sandbox|Project sandbox: This is your playground (everybody can use it)]]
 
[[/project_template|Project template: Reference template for future classes (please do not modify)]]
 
 
[[/project00|Project 00: Title of your project and "team name"]]
 
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Latest revision as of 16:31, 22 March 2013

840:153g: Recombinant DNA Te(a)chniques

Home        People        Projects        Materials        Schedule        Help       

2012 (Fall) Projects

Project 27: Expression of methane monooxygenase in E. coli for biodegradation of methane and trichloroethylene. By Austin Jones and Jace Dolphin

Project 26: The Cloning of Atrolysin A from Crotalus atrox and "Stone Cold" and Kayla Ohrt & AJ Goos

Project 25: Cloning of MreB from Caulobacter crescentus into E. coli. "Tango-Alpha" (Colby Swanson & Bess Lippmann)

Project 24: Over expression of Acetyl- CoA carboxylase (ACC) sub-unit accC in E.coli to enhance fatty acid accumulation for Bio-fuel production”."Fuel-it-up" (Sanju Timilsina and Parul Sirohi)

Project 23: Cloning of the Outer Membrane f-type Cytochrome (OmcF) gene for the production of Bioelectricity by Valerie Wisco and Casey Durnan

Project 22: Production of prodigiosin in E. Coli by Team Jacob (Stephanie Vondrak, Brian Hovey)

Project 21: Cloning of PMT1149 antifreeze gene into E. Coli "Frozen Assets" (Brandon Ramirez and Serge Marraback)

2011 (Fall) Projects

Project 20: Cloning and expression of neutral protease gene from B. Stearothermophilus / (TIC)

Project 19: Cloning tps1 gene in Citrus Sinensis/ Orange you glad we didn't say Banana? (Beth, Cassidy, OJ)

Project 18: Cloning the gene of Acenitobacter calcoaceticus strain ADP1 to observe its expression in E.coli / GAS

Project 17: Cloning the Progesterone 5-beta Reductase Gene from Selaginella moellendorffii (Charlie, Sheree, Amanda)

Project 15: G.tigrina Hox Gene Dthox C Insertion Into Prokaryote E.coli / Team UNIamCloning (Alex, Courtney)

Project 14: Cloning of the Orcinol O-methyltransferase (OOMT2) Gene in Roses (Megan, Kim)

2010 (Fall) Projects

Project 13: Detecting Estrogen in Water with Florescence "Team Detectors"

Project 12: Oscillating fluorescence in E. coli and "Team BAs"

Project 11: E. coli fluorescing based on cold temp sensor and "E cool I"

Project 10: Remediation of Phenol Resin using White Rot LiP transferred into E. Coli and "team Fun Guys"

Project 9: Tetrodotoxin Production in E. coli Using Pufferfish Flp Genes. "Team Petrificus Totalus"

2009 (Fall) Projects

Project 8: Human Factor X: The NeXt generation of clotting

Project 7: E. coli Tic Tacs

Project 6: Strawberry Shortcake: An Exploration of the FaQR Gene

2009 (Spring) Projects

Project 5: NarL promotor manipulation "g(NarL)y prom(-O)ters"

Project 4: Anti-Fungal Bacteria "MoldBusters"

Project 3: Tear Inducing Bacteria by "It's my E. coli and I'll cry if I want to"

2008 (Fall) Projects

Project 2: The sweet smell of ...E.coli?

Project 1: Our Field of Dreams by "Emblazon"


Templates (please don't delete)

Project sandbox: This is your playground (everybody can use it)

Project template: Reference template for future classes (please do not modify)


Project 00: Title of your project and "team name"

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