840:153g:Projects/project1/2008/10/14: Difference between revisions
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== | ==DNA Purication and Vector extraction== | ||
A Flower of A Different Color | A Flower of A Different Color | ||
The DNA group (Rajiv, Tiffany and Melissa) discused with Axel about the next step for the DNA PCR product and RNA extraction procedures. We came to a conclusion that the DNA PCR Product will be used to be sequenced. To do so we will have to purify the DNA PCR product using the verbal protocol from Axel on DNA purification. The procedure is as stated: Combine all PCR products that amplified, samples C5, C6, E5, E8, and E9 into one centrifuge tube. Next we added .6 times the volume of the PCR sample PEG with 2.5M NaCl. Then incubated at room temperature for 15 minutes and centrifuge for 30 minutes at 15,000X. the supernant was dicarded and the clear pellet was brought back up to volume with 20 mircoliters of diH2O. To test the amount of actual DNA present in teh sample we used Electrophoresis to test the sample. | |||
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Revision as of 13:46, 14 October 2008
 A Flower of a Different Color by "Emblazon"
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DNA Purication and Vector extractionA Flower of A Different Color The DNA group (Rajiv, Tiffany and Melissa) discused with Axel about the next step for the DNA PCR product and RNA extraction procedures. We came to a conclusion that the DNA PCR Product will be used to be sequenced. To do so we will have to purify the DNA PCR product using the verbal protocol from Axel on DNA purification. The procedure is as stated: Combine all PCR products that amplified, samples C5, C6, E5, E8, and E9 into one centrifuge tube. Next we added .6 times the volume of the PCR sample PEG with 2.5M NaCl. Then incubated at room temperature for 15 minutes and centrifuge for 30 minutes at 15,000X. the supernant was dicarded and the clear pellet was brought back up to volume with 20 mircoliters of diH2O. To test the amount of actual DNA present in teh sample we used Electrophoresis to test the sample. | |
