840:153g:Projects/project10/2010/10/28

From OpenWetWare

< 840:153g:Projects | project10 | 2010 | 10(Difference between revisions)
Jump to: navigation, search
(Autocreate 2010/10/28 Entry for 840:153g:Projects/project10)
Current revision (15:23, 28 October 2010) (view source)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Entry title==
-
* Insert content here...
+
* Tuesday, attempted RNA extraction for the second time using all RNase free materials this time. This time, the sample had a gelatinous consistency that was difficult to pipette - we lost some sample. Thursday set up and ran RT-PCR using the same primer and control sets as before (Grumpy+Cowgirl and Sneezy+Cowgirl). Will do agarose gel electrophoresis to see if we successfully extracted RNA. If the gel shows no presence of cDNA (from RNA in the RT-PCR), we will run RNA gels on our extraction samples from both attempts.

Current revision

Project name Main project page
Previous entry      Next entry

Entry title

  • Tuesday, attempted RNA extraction for the second time using all RNase free materials this time. This time, the sample had a gelatinous consistency that was difficult to pipette - we lost some sample. Thursday set up and ran RT-PCR using the same primer and control sets as before (Grumpy+Cowgirl and Sneezy+Cowgirl). Will do agarose gel electrophoresis to see if we successfully extracted RNA. If the gel shows no presence of cDNA (from RNA in the RT-PCR), we will run RNA gels on our extraction samples from both attempts.


Personal tools