840:153g:Projects/project10/2010/11/04: Difference between revisions
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==Entry title== | ==Entry title== | ||
Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR.[[Image:RT Two.jpg]] | Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR. First two lanes are G & C primers, middle is ladder, and last two lanes are S &C primers. [[Image:RT Two.jpg]] | ||
Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded. | Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded. | ||
Revision as of 14:49, 4 November 2010
 Project name
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Entry titleDiscovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR. First two lanes are G & C primers, middle is ladder, and last two lanes are S &C primers.
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