840:153g:Projects/project10/2010/12/02 - Revision history

Lorena Christensen at 21:27, 2 December 2010

Lorena Christensen — Thu, 02 Dec 2010 21:27:53 GMT

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	* Tuesday, we used our 10 plasmid minipreps and cut them with EcoR1 and ran gel electrophoresis on them to see the sizes of fragments. We discovered only a few of our samples turned out with two bands, one at about 3,000bp and the other at 900 bp. The 3,000bp fragment is thought to be the plasmid, and the 900bp is our gene of interest insertion. Well #21 is showing a band at ~600bp and ~100bp. We sent four samples to be sequenced (wells 18, 19, 21, and 22). Thursday, we did clean up and saved some samples for future use.		* Tuesday, we used our 10 plasmid minipreps and cut them with EcoR1 and ran gel electrophoresis on them to see the sizes of fragments. We discovered only a few of our samples turned out with two bands, one at about 3,000bp and the other at 900 bp. The 3,000bp fragment is thought to be the plasmid, and the 900bp is our gene of interest insertion. Well #21 is showing a band at ~600bp and ~100bp. We sent four samples to be sequenced (wells 18, 19, 21, and 22). Thursday, we did clean up and saved some samples for future use.

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Lorena Christensen at 21:25, 2 December 2010

Lorena Christensen — Thu, 02 Dec 2010 21:25:54 GMT

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-	* ~~Insert content here~~...	+	* Tuesday, we used our 10 plasmid minipreps and cut them with EcoR1 and ran gel electrophoresis on them to see the sizes of fragments. We discovered only a few of our samples turned out with two bands, one at about 3,000bp and the other at 900 bp. The 3,000bp fragment is thought to be the plasmid, and the 900bp is our gene of interest insertion. Well #21 is showing a band at ~600bp and ~100bp. We sent four samples to be sequenced (wells 18, 19, 21, and 22). Thursday, we did clean up and saved some samples for future use.
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Lorena Christensen: Autocreate 2010/12/02 Entry for 840:153g:Projects/project10

Lorena Christensen — Thu, 02 Dec 2010 21:06:17 GMT

Autocreate 2010/12/02 Entry for 840:153g:Projects/project10

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{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__
[[category:OWWLabNotebookV1]]