840:153g:Projects/project11/2010/09/16

From OpenWetWare

< 840:153g:Projects | project11 | 2010 | 09(Difference between revisions)
Jump to: navigation, search
(Autocreate 2010/09/16 Entry for 840:153g:Projects/project11)
Current revision (16:26, 16 September 2010) (view source)
(Entry title)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Entry title==
-
* Insert content here...
+
* Preparing media and isolating plasmid with gene.
 +
 
 +
Prepared LB agar and stored overnight in refrigerator on Tuesday. Thursday we autoclaved the agar, poured two control plates, added antibiotics. KAN to 700ml of agar in one flask. Added X Gal, IPPG, and Ampicilin (each 800ul) to the other agar flask. Poured plates, let sit, and stored. Thursday we also isolated our plasmid containing our gene and stored in freezer.  

Current revision

Project name Main project page
Next entry

Entry title

  • Preparing media and isolating plasmid with gene.

Prepared LB agar and stored overnight in refrigerator on Tuesday. Thursday we autoclaved the agar, poured two control plates, added antibiotics. KAN to 700ml of agar in one flask. Added X Gal, IPPG, and Ampicilin (each 800ul) to the other agar flask. Poured plates, let sit, and stored. Thursday we also isolated our plasmid containing our gene and stored in freezer.


Personal tools