- Tuesday we transformed bacteria and plated the transformed bacteria. Isolated other plasmids (RBS, Promoter, and Double Terminator using same protocol as isolating gene plasmid from well plate. Froze the isolated plasmids. Wednesday we took transformed colonies from plates and placed the colonies into their own separate overnight LB with antibiotic stock to continue growth. Thursday we isolated plasmids from E. coli using the Gene Jet Mini Prep Kit. Froze isolated plasmids at -80 degrees celsius. Made glycerol stocks for left over transformed bacteria for long term storage and stored at -80 degrees celsius. Went over digestion and gel electrophoresis procedure for Tuesday.