840:153g:Projects/project11/2010/09/30: Difference between revisions
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* Tuesday we digested DNA from our 11 samples and 1 sample of BLUESCRIPT plasmid (control). Performed gel electrophoresis to ensure correct part based on base pair length. Took photo of result with UV camera. | * Tuesday we digested DNA from our 11 samples and 1 sample of BLUESCRIPT plasmid (control). Performed gel electrophoresis to ensure correct part based on base pair length. Took photo of result with UV camera. | ||
Thursday we performed another digestion with DNA from sample 1 which had the best band resolution from the photo of the previous gel electrophoresis. After digestion, we ran it on another gel separated into four wells. Took photo of that gel and cut out the bands at around seven hundred base pairs. Separated into two tubes and weighed them individually. Stored at -80C. | Thursday we performed another digestion with DNA from sample 1 which had the best band resolution from the photo of the previous gel electrophoresis. After digestion, we ran it on another gel separated into four wells. Took photo of that gel and cut out the bands at around seven hundred base pairs. Separated into two tubes and weighed them individually. Stored at -80C. | ||
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Revision as of 14:32, 30 September 2010
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Entry title
Thursday we performed another digestion with DNA from sample 1 which had the best band resolution from the photo of the previous gel electrophoresis. After digestion, we ran it on another gel separated into four wells. Took photo of that gel and cut out the bands at around seven hundred base pairs. Separated into two tubes and weighed them individually. Stored at -80C. | |
