840:153g:Projects/project11/2010/09/30

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  • Tuesday we digested DNA from our 11 samples and 1 sample of BLUESCRIPT plasmid (control). Performed gel electrophoresis to ensure correct part (gene) based on base pair length. Took photo of result with UV camera.

Thursday we performed another digestion with DNA from sample 1 which had the best band resolution from the photo of the previous gel electrophoresis. After digestion, we ran it on another gel separated into four wells. Took photo of that gel and cut out the bands at around seven hundred base pairs. Separated into two tubes and weighed them individually. Stored at -80C. Next week we plan on transforming bacteria with all of our other parts that are in plasmids and then isolating the plasmids from the E. coli.

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