840:153g:Projects/project11/2010/10/14: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 10: | Line 10: | ||
Thursday we received our parts and calculated amount of water to add to each to make them 100umol. Made two working stocks from those two oligos (TUNA and tintin) that were of 10umol concentration. We also purified our gene (BBa_E1010) from the gel on 9/30/10 using the Gel Extraction Kit. Froze the extracted gene DNA in freezer. | Thursday we received our parts and calculated amount of water to add to each to make them 100umol. Made two working stocks from those two oligos (TUNA and tintin) that were of 10umol concentration. We also purified our gene (BBa_E1010) from the gel on 9/30/10 using the Gel Extraction Kit. Froze the extracted gene DNA in freezer. | ||
Next week we plan on running PCR on the oligos to combine the two oligos. We may start ligation of our parts on Thursday if time permits. | Next week we plan on running PCR on the oligos to combine the two oligos. We may start ligation of our parts on Thursday if time permits. | ||
http://www.openwetware.org/wiki/Image:Gel_from_10_12.JPG#filelinks | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 14:12, 14 October 2010
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry title
Thursday we received our parts and calculated amount of water to add to each to make them 100umol. Made two working stocks from those two oligos (TUNA and tintin) that were of 10umol concentration. We also purified our gene (BBa_E1010) from the gel on 9/30/10 using the Gel Extraction Kit. Froze the extracted gene DNA in freezer. Next week we plan on running PCR on the oligos to combine the two oligos. We may start ligation of our parts on Thursday if time permits. http://www.openwetware.org/wiki/Image:Gel_from_10_12.JPG#filelinks |