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Thursday we received our parts and calculated amount of water to add to each to make them 100umol. Made two working stocks from those two oligos (TUNA and tintin) that were of 10umol concentration. We also purified our gene (BBa_E1010) from the gel on 9/30/10 using the Gel Extraction Kit. Froze the extracted gene DNA in freezer. Next week we plan on running PCR on the oligos to combine the two oligos. We may start ligation of our parts on Thursday if time permits.