840:153g:Projects/project11/2010/10/28

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(Autocreate 2010/10/28 Entry for 840:153g:Projects/project11)
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==Entry title==
==Entry title==
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* Tuesday we digested our Promoter(BBa_I2007) with Spe1. It was then purified using the GeneJET PCR purification kit. Ligated Promoter with the RBS that we created from oligos. We also ligated the gene(BBa_E1010) to the double terminator (BBa_B0015). Store in refrigerator overnight.
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Thursday we transformed E.coli with the ligated parts. Plated the trasnformed bacteria onto antibiotic plates. We plan to incubate overnight, then store in refrigerator until Monday when we will grow liquid cultures. During next week, we will isolate plasmids, cut and see if parts were ligated correctly by running on a gel. 

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  • Tuesday we digested our Promoter(BBa_I2007) with Spe1. It was then purified using the GeneJET PCR purification kit. Ligated Promoter with the RBS that we created from oligos. We also ligated the gene(BBa_E1010) to the double terminator (BBa_B0015). Store in refrigerator overnight.

Thursday we transformed E.coli with the ligated parts. Plated the trasnformed bacteria onto antibiotic plates. We plan to incubate overnight, then store in refrigerator until Monday when we will grow liquid cultures. During next week, we will isolate plasmids, cut and see if parts were ligated correctly by running on a gel.


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