840:153g:Projects/project11/2010/11/04: Difference between revisions
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Monday: counted colonies from transformed bacteria plated last thursday. prepared media for onvernight growth, scraped of single colonies and placed into media. | Monday: counted colonies from transformed bacteria plated last thursday. prepared media for onvernight growth, scraped of single colonies and placed into media. | ||
10 tubes of superpart (promoter & rbs) | 10 tubes of superpart (promoter & rbs) | ||
5 tubes- superpart (gene & double terminator). we took more of the first superpart due to concerns with ligation oriantation | 5 tubes- superpart (gene & double terminator). we took more of the first superpart due to concerns with ligation oriantation. | ||
Incubated at 37 degrees overnight to grow liquid cultures. | Incubated at 37 degrees overnight to grow liquid cultures. | ||
Revision as of 14:00, 4 November 2010
 Project name
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Entry titleMonday: counted colonies from transformed bacteria plated last thursday. prepared media for onvernight growth, scraped of single colonies and placed into media. 10 tubes of superpart (promoter & rbs) 5 tubes- superpart (gene & double terminator). we took more of the first superpart due to concerns with ligation oriantation. Incubated at 37 degrees overnight to grow liquid cultures. tuesday: plasmid mini prep to isolate plasmid from bacteria in preperation for digestion and Gel eletrophoresis. Thursday: digsted superpart promoter & rbs with EcoR1, Spe1 Superpart2 gene & double terminator with xball, pst1. ran on gel to confirm proper oriantation and base pair length of ligated parts. took picture next week plan to ligated the 2 confirmed superparts together. forming our completed part | |
