840:153g:Projects/project12/2010/11/18

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(Autocreate 2010/11/18 Entry for 840:153g:Projects/project12)
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==Entry title==
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Disappointment
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* On Tuesday, we digested our lux superpart (BBa_J37015) and ran on a gel with our PCR products of the RFP protein. We didn't see the two bands at around 2600 bp and 2000 bp for the lux superpart, but we did see the amplification of our RFP protein. So we had the lux superpart sent in to be sequenced. Today, we did a digestion of the lux promoter and ran a gel at 1.7%. We saw faint bands, but not where we expect them to be. So, we threw the gel away. We are having two different promoter samples sent in to be sequenced as well. 

Revision as of 19:09, 18 November 2010

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Disappointment

  • On Tuesday, we digested our lux superpart (BBa_J37015) and ran on a gel with our PCR products of the RFP protein. We didn't see the two bands at around 2600 bp and 2000 bp for the lux superpart, but we did see the amplification of our RFP protein. So we had the lux superpart sent in to be sequenced. Today, we did a digestion of the lux promoter and ran a gel at 1.7%. We saw faint bands, but not where we expect them to be. So, we threw the gel away. We are having two different promoter samples sent in to be sequenced as well.


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