840:153g:Projects/project16/2011/09/15

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(Autocreate 2011/09/15 Entry for 840:153g:Projects/project16)
Current revision (19:26, 22 September 2011) (view source)
(Chelex DNA Extraction)
 
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==Entry title==
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==Chelex Extraction of DNA==
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We elected to use a Chelex extraction protocol to extract Kelsely's DNA from the cells. The cells were obtained from the inner cheek of Kelsey's mouth. The sample was incubated, vortexed, and centrifuged to separate DNA from the Chelex molecules. The Chelex lyses the cells and binds to ions to prevents a large number of ions from binding to the negatively charged DNA. In our next lab session, we plan to prepare an agarose gel electrophoresis and run our DNA to check the effectiveness of our Chelex extraction. Depending on the outcome of our gel electrophoresis, we will either proceed to purifying our DNA or performing a new DNA extraction with a different protocol.

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Chelex Extraction of DNA

We elected to use a Chelex extraction protocol to extract Kelsely's DNA from the cells. The cells were obtained from the inner cheek of Kelsey's mouth. The sample was incubated, vortexed, and centrifuged to separate DNA from the Chelex molecules. The Chelex lyses the cells and binds to ions to prevents a large number of ions from binding to the negatively charged DNA. In our next lab session, we plan to prepare an agarose gel electrophoresis and run our DNA to check the effectiveness of our Chelex extraction. Depending on the outcome of our gel electrophoresis, we will either proceed to purifying our DNA or performing a new DNA extraction with a different protocol.


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