From OpenWetWare

< 840:153g:Projects | project16/2011 | 09
Revision as of 19:24, 22 September 2011 by Kelsey Hampton (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

DNA Purification through Phenol/Chloroform Extraction and Ethanol Precipitation

Today we located two protocols for DNA purificaiton, through phenol/chloroform extraction and ethanol precipitation. We separated our sample of crude DNA into three test tubes in 100 microliter increments to attempt one phenol/chloroform extraction, one ethanol precipitation, and one phenol/chloroform extraction followed by an ethanol precipitation. One of the phenol/chloroform extractions went awry, inverting the organic and aqueous layers. This, coupled with the accidental discarding of the supposed organic layer, caused us to only do two experiments: one with the phenol/chloroform extraction only, the other with the phenol/chloroform extraction and ethanol precipitation. The extractions were finished, and 2.5 volumes of ethanol (200 microliters) in tube 1. The samples were frozen.

Personal tools