840:153g:Projects/project16/2011/09/29

From OpenWetWare

< 840:153g:Projects | project16/2011 | 09(Difference between revisions)
Jump to: navigation, search
(Autocreate 2011/09/29 Entry for 840:153g:Projects/project16)
Current revision (10:58, 30 September 2011) (view source)
(Initial PCR)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==PCR==
-
* Insert content here...
+
Today we set up a PCR for our two DNA samples; the phenol/chloroform extraction and the phenol/chloroform coupled with ethanol precipitation. The negative control consisted of only primers in the PCR mix and the positive controls were plasmid DNA with plasmid primers and plasmid primers by themselves. We chose the temperatures of 43 C, 48 C and 52.1 C The results of this will determine if our method for DNA extraction will be effective to proceed in our project.  

Current revision

Project name Main project page
Previous entry      Next entry

PCR

Today we set up a PCR for our two DNA samples; the phenol/chloroform extraction and the phenol/chloroform coupled with ethanol precipitation. The negative control consisted of only primers in the PCR mix and the positive controls were plasmid DNA with plasmid primers and plasmid primers by themselves. We chose the temperatures of 43 C, 48 C and 52.1 C The results of this will determine if our method for DNA extraction will be effective to proceed in our project.


Personal tools