840:153g:Projects/project16/2011/10/04: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2011/10/04 Entry for 840:153g:Projects/project16) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Gel Electrophoresis of PCR and Creating Lysis Buffer== | ||
Today, we ran our PCR products through a gel to determine if we successfully amplified our gene. Unfortunately, we failed to amplify our gene and we forgot to add the master mix to 2 of our 3 controls. There are many different things that could have made our PCR failed which include a failed DNA extraction, incorrect annealing temperatures, an incorrect master mix, etc. So we elected to try a different extraction protocol that we acquired from Dr. Spradling. In preparation for Thursday's lab, we created our lysis buffer. | |||
Revision as of 15:37, 4 October 2011
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Gel Electrophoresis of PCR and Creating Lysis BufferToday, we ran our PCR products through a gel to determine if we successfully amplified our gene. Unfortunately, we failed to amplify our gene and we forgot to add the master mix to 2 of our 3 controls. There are many different things that could have made our PCR failed which include a failed DNA extraction, incorrect annealing temperatures, an incorrect master mix, etc. So we elected to try a different extraction protocol that we acquired from Dr. Spradling. In preparation for Thursday's lab, we created our lysis buffer.
| |
