840:153g:Projects/project16/2011/10/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Gel Electrophoresis of PCR Products from (10/13/2011)==
* Today we did a gel electrophoresis of our PCR products from the last session to determine if we successfully amplified our gene.  We messed up two gels before finally getting it right the third time.
* Today we did a gel electrophoresis of our PCR products from the last session to determine if we successfully amplified our gene.  We messed up two gels before finally getting it right the third time.  Jack wasn't paying good enough attention and ended up adding two of our PCR products in the same lane.  To add insult to injury, our gel failed miserably as our negative control turned up positive and none of the DNA we sequenced was anywhere close to what we expected.  Therefore, we have elected to redo the PCR procedure from 10/11/2011 because that at least worked and use tubes to scrape the DNA from the right band.





Latest revision as of 21:01, 26 September 2017

Project name Main project page
Previous entry      Next entry

Gel Electrophoresis of PCR Products from (10/13/2011)

  • Today we did a gel electrophoresis of our PCR products from the last session to determine if we successfully amplified our gene. We messed up two gels before finally getting it right the third time. Jack wasn't paying good enough attention and ended up adding two of our PCR products in the same lane. To add insult to injury, our gel failed miserably as our negative control turned up positive and none of the DNA we sequenced was anywhere close to what we expected. Therefore, we have elected to redo the PCR procedure from 10/11/2011 because that at least worked and use tubes to scrape the DNA from the right band.