840:153g:Projects/project17/2011/09/22

Today in lab we extracted DNA from our plant. (which has arrived!!!) We crushed up a few leaves of it in some liquid nitrogen and ground it into a very fine powder which we then placed into tubes and used the protocol for plants from Qiagen Bench protocol: DNeasy plant mini. We also prepared our 10 µmolar dilutions of primers and stored them in the -80 °C freezer.

In the next lab we plan to make some gel to run an electrophoresis to make sure that we isolated our DNA. If our promoter parts come we can make stocks of them too. Or possibly do some PCR on our gene.