840:153g:Projects/project20/2011/10/20

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(Entry title)
Current revision (14:29, 8 December 2011) (view source)
(Agarose gel electrophoresis (PCR: 2nd Attempt))
 
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==Entry title==
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==Plasmid Extraction from Transformed competent cells==
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* Plamid exatraction from overnight incubated transformed ''e.coli'' plates
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* Plasmid extraction from overnight incubated transformed ''e.coli'' plates
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== Agarose gel electrophoresis (PCR: 2nd Attempt)==
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* Results very familiar to 1st attempt of PCR.
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==PCR(3rd Attempt==
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* On the results on 2nd PCR attempt.We decide to next gradient pcr for our nprT Gene at higher temperature(above 48 degree Celsius)
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Plasmid Extraction from Transformed competent cells

  • Plasmid extraction from overnight incubated transformed e.coli plates

Agarose gel electrophoresis (PCR: 2nd Attempt)

  • Results very familiar to 1st attempt of PCR.

PCR(3rd Attempt

  • On the results on 2nd PCR attempt.We decide to next gradient pcr for our nprT Gene at higher temperature(above 48 degree Celsius)
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