840:153g:Projects/project20/2011/11/01

(Difference between revisions)

Revision as of 23:36, 1 November 2011

Project name

After 3 gradient PCR attempts, We are unable to get proper amplification i.e single cut clear band on the agarose gel.
We were trying to clone the PCR product containing segment which has wrongly amplified.
For cloning, we have to purify PCR product.So initially we run 13 samples on gel and then purify using Kit based method.
We also directly purify the 3 PCR product without running on a gel using Kit based method.
Sample classification depend upon intensity of band appear previously on gel.

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+* After 3 gradient PCR attempts, We are unable to get proper amplification i.e single cut clear band on the agarose gel.
+* We were trying to clone the PCR product containing segment which has wrongly amplified.
+* For cloning, we have to purify PCR product.So initially we run 13 samples on gel and then purify using Kit based method.
+* We also directly purify the 3 PCR product without running on a gel using Kit based method.
+* Sample classification depend upon intensity of band appear previously on gel.