840:153g:Projects/project20/2011/11/01: Difference between revisions
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==Entry title== | ==Entry title== | ||
* | * After 3 gradient PCR attempts, We are unable to get proper amplification i.e single cut clear band on the agarose gel. | ||
* We were trying to clone the PCR product containing segment which has wrongly amplified. | |||
* For cloning, we have to purify PCR product.So initially we run 13 samples on gel and then purify using Kit based method. | |||
* We also directly purify the 3 PCR product without running on a gel using Kit based method. | |||
* Sample classification depend upon intensity of band appear previously on gel. | |||
Revision as of 20:36, 1 November 2011
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