840:153g:Projects/project20/2011/11/01: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==Entry title==
==PCR Product Purification==
* After 3 gradient PCR attempts, We are unable to get proper amplification i.e single cut clear band on the agarose gel.
* After 3 gradient PCR attempts, We are unable to get proper amplification i.e single cut clear band on the agarose gel.
* We were trying to clone the PCR product containing segment which has wrongly amplified.
* We were trying to clone the PCR product containing segment which has wrongly amplified.

Latest revision as of 21:04, 26 September 2017

Project name Main project page
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PCR Product Purification

  • After 3 gradient PCR attempts, We are unable to get proper amplification i.e single cut clear band on the agarose gel.
  • We were trying to clone the PCR product containing segment which has wrongly amplified.
  • For cloning, we have to purify PCR product.So initially we run 13 samples on gel and then purify using Kit based method.
  • We also directly purify the 3 PCR product without running on a gel using Kit based method.
  • Sample classification depend upon intensity of band appear previously on gel.


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