840:153g:Projects/project20/2011/11/01 - Revision history

Srikanth Sistlas: /* Entry title */

2011-12-08T18:29:42Z

Entry title

←Older revision		Revision as of 18:29, 8 December 2011
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-	==~~Entry title~~==	+	==PCR Product Purification==
	* After 3 gradient PCR attempts, We are unable to get proper amplification i.e single cut clear band on the agarose gel.		* After 3 gradient PCR attempts, We are unable to get proper amplification i.e single cut clear band on the agarose gel.
	* We were trying to clone the PCR product containing segment which has wrongly amplified.		* We were trying to clone the PCR product containing segment which has wrongly amplified.

Srikanth Sistlas: /* Entry title */

2011-11-02T03:36:24Z

Entry title

←Older revision		Revision as of 03:36, 2 November 2011
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	==Entry title==		==Entry title==
-	* ~~Insert content here~~...	+	* After 3 gradient PCR attempts, We are unable to get proper amplification i.e single cut clear band on the agarose gel.
		+	* We were trying to clone the PCR product containing segment which has wrongly amplified.
		+	* For cloning, we have to purify PCR product.So initially we run 13 samples on gel and then purify using Kit based method.
		+	* We also directly purify the 3 PCR product without running on a gel using Kit based method.
		+	* Sample classification depend upon intensity of band appear previously on gel.

Srikanth Sistlas: Autocreate 2011/11/01 Entry for 840:153g:Projects/project20

2011-11-02T03:20:50Z

Autocreate 2011/11/01 Entry for 840:153g:Projects/project20

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
* Insert content here...


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