840:153g:Projects/project20/2011/11/10
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< 840:153g:Projects | project20 | 2011 | 11(Difference between revisions)
(Autocreate 2011/11/10 Entry for 840:153g:Projects/project20) |
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| - | == | + | ==Ligation Trial 2== |
| - | * | + | * Transformation experiment done on 11/08/11 failed. |
| + | * We decide to increase the concentration of DNA in Ligation reaction. | ||
| + | * We increse concentration to 25 times. It means 1 part of vector and 25 parts of insert. | ||
| + | * After completing experiment, we realized that for first ligation experiment we have frozen ligation mix.So thats why ligation trial 1 failed. | ||
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Ligation Trial 2
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