840:153g:Projects/project22/2012/10/04: Difference between revisions
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== | ==PCR Results and Retry== | ||
On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few number of DNA samples to make sure our controls work properly before we go all in with the rest of our primers. | On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few number of DNA samples to make sure our controls work properly before we go all in with the rest of our primers. | ||
Revision as of 13:01, 4 October 2012
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PCR Results and RetryOn Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few number of DNA samples to make sure our controls work properly before we go all in with the rest of our primers.
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