840:153g:Projects/project22/2012/10/04 - Revision history

Brian Hovey: /* PCR Results and Retry */

2012-10-18T23:48:22Z

PCR Results and Retry

←Older revision		Revision as of 23:48, 18 October 2012
Line 10:		Line 10:
	On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA on Tuesday to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR next week. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in with our biobrick and nonbiobrick compatible primers.		On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA on Tuesday to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR next week. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in with our biobrick and nonbiobrick compatible primers.

-	Failed PCR picture 1	+	Failed PCR picture 1 (positive controls, PCR and marker - only the marker showed up with any significant brightness)
	[[Image:!GROUP22.25_(2).png]]		[[Image:!GROUP22.25_(2).png]]

Brian Hovey: /* PCR Results and Retry */

2012-10-18T23:47:38Z

PCR Results and Retry

←Older revision		Revision as of 23:47, 18 October 2012
Line 10:		Line 10:
	On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA on Tuesday to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR next week. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in with our biobrick and nonbiobrick compatible primers.		On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA on Tuesday to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR next week. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in with our biobrick and nonbiobrick compatible primers.

-	~~Wells~~ 1~~-12 were ours (markers at 5 and 12 going right to left)~~	+	Failed PCR picture 1
	[[Image:!GROUP22.25_(2).png]]		[[Image:!GROUP22.25_(2).png]]

-	~~Wells 18-20 were ours~~ (negative controls and marker) - Note that one of our negative controls was excluded from this picture	+	Failed PCR picture 2 (negative controls and marker) - Note that one of our negative controls was excluded from this picture
	[[Image:!GROUP24.2_(2).png]]		[[Image:!GROUP24.2_(2).png]]

Brian Hovey: /* PCR Results and Retry */

2012-10-18T23:47:03Z

PCR Results and Retry

←Older revision		Revision as of 23:47, 18 October 2012
Line 11:		Line 11:

	Wells 1-12 were ours (markers at 5 and 12 going right to left)		Wells 1-12 were ours (markers at 5 and 12 going right to left)
-	[~~http~~:~~//openwetware~~.~~org/images/thumb/4/44/%21GROUP22~~.~~25O.tif/640px~~-~~%21GROUP22.25O~~.~~tif~~.png]	+	[[Image:!GROUP22.25_(2).png]]
		+
		+	Wells 18-20 were ours (negative controls and marker) - Note that one of our negative controls was excluded from this picture
		+	[[Image:!GROUP24.2_(2).png]]

-	~~Wells 18-20 were ours (negative controls and marker, far left side)~~
-	~~[http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.tif.png]~~

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Brian Hovey: /* PCR Results and Retry */

2012-10-04T22:31:08Z

PCR Results and Retry

←Older revision		Revision as of 22:31, 4 October 2012
Line 8:		Line 8:

	==PCR Results and Retry==		==PCR Results and Retry==
-	On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA on Tuesday to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR next week. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in.	+	On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA on Tuesday to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR next week. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in with our biobrick and nonbiobrick compatible primers.

	Wells 1-12 were ours (markers at 5 and 12 going right to left)		Wells 1-12 were ours (markers at 5 and 12 going right to left)

Brian Hovey: /* PCR Results and Retry */

2012-10-04T22:30:25Z

PCR Results and Retry

←Older revision		Revision as of 22:30, 4 October 2012
Line 8:		Line 8:

	==PCR Results and Retry==		==PCR Results and Retry==
-	On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in.	+	On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA on Tuesday to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR next week. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in.

	Wells 1-12 were ours (markers at 5 and 12 going right to left)		Wells 1-12 were ours (markers at 5 and 12 going right to left)

Brian Hovey: /* PCR Results and Retry */

2012-10-04T22:29:20Z

PCR Results and Retry

←Older revision		Revision as of 22:29, 4 October 2012
Line 13:		Line 13:
	[http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.tif.png]		[http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.tif.png]

-	Wells 18-20 were ours (negative controls, far left side)	+	Wells 18-20 were ours (negative controls and marker, far left side)
	[http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.tif.png]		[http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.tif.png]

Brian Hovey: /* PCR Results and Retry */

2012-10-04T22:26:45Z

PCR Results and Retry

←Older revision		Revision as of 22:26, 4 October 2012
Line 10:		Line 10:
	On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in.		On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in.

-	Wells 1-12 were ours	+	Wells 1-12 were ours (markers at 5 and 12 going right to left)
	[http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.tif.png]		[http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.tif.png]

-	Wells 19-20 were ours (negative controls)	+	Wells 18-20 were ours (negative controls, far left side)
	[http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.tif.png]		[http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.tif.png]

Brian Hovey: /* PCR Results and Retry */

2012-10-04T22:25:52Z

PCR Results and Retry

←Older revision		Revision as of 22:25, 4 October 2012
Line 11:		Line 11:

	Wells 1-12 were ours		Wells 1-12 were ours
-	[~~[Image:~~http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.tif.png]]	+	[http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.tif.png]

	Wells 19-20 were ours (negative controls)		Wells 19-20 were ours (negative controls)
-	[~~[Image:~~http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.tif.png]]	+	[http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.tif.png]

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Brian Hovey at 22:24, 4 October 2012

2012-10-04T22:24:54Z

←Older revision		Revision as of 22:24, 4 October 2012
Line 11:		Line 11:

	Wells 1-12 were ours		Wells 1-12 were ours
-	[[Image:http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.png]]	+	[[Image:http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.tif.png]]

	Wells 19-20 were ours (negative controls)		Wells 19-20 were ours (negative controls)
-	[[Image:http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.png]]	+	[[Image:http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.tif.png]]

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Brian Hovey: /* PCR Results and Retry */

2012-10-04T20:20:15Z

PCR Results and Retry

←Older revision		Revision as of 20:20, 4 October 2012
Line 11:		Line 11:

	Wells 1-12 were ours		Wells 1-12 were ours
-	[[Image:http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O~~.tif~~.png]]	+	[[Image:http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.png]]

	Wells 19-20 were ours (negative controls)		Wells 19-20 were ours (negative controls)
-	[[Image:http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2~~.tif~~.png]]	+	[[Image:http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.png]]

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->