840:153g:Projects/project22/2012/10/11

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(Autocreate 2012/10/11 Entry for 840:153g:Projects/project22)
Current revision (15:12, 16 October 2012) (view source)
(Confirmation of DNA content)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Prodigiosin Production in E.Coli</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Confirmation of DNA content==
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* Insert content here...
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* On Tuesday, we ran gel electrophoresis on our now concentrated genomic DNA samples. Only sample 3 (from samples 1, 2, 3, and 5) produced a visible band. We did not feel confident that this was DNA, so today we ran digested and undigested samples from sample 3 to confirm its DNA content before we proceed. If sample 3 shows that it is liable to digestion, we will proceed with PCR. If not, we will return to our other samples and may also extract DNA from ''Serratia marcescens'' with a new extraction protocol.

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Confirmation of DNA content

  • On Tuesday, we ran gel electrophoresis on our now concentrated genomic DNA samples. Only sample 3 (from samples 1, 2, 3, and 5) produced a visible band. We did not feel confident that this was DNA, so today we ran digested and undigested samples from sample 3 to confirm its DNA content before we proceed. If sample 3 shows that it is liable to digestion, we will proceed with PCR. If not, we will return to our other samples and may also extract DNA from Serratia marcescens with a new extraction protocol.


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