840:153g:Projects/project22/2012/10/11 - Revision history

Stephanie Vondrak: /* Confirmation of DNA content */

Stephanie Vondrak — Tue, 16 Oct 2012 19:12:18 GMT

Confirmation of DNA content

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	==Confirmation of DNA content==		==Confirmation of DNA content==
-	* On Tuesday, we ran gel electrophoresis on our now concentrated DNA samples. Only sample 3 (from samples 1, 2, 3, and 5) ~~showed significant protein content~~. We did not feel confident that this ~~protein product~~ was DNA, so today we ran digested and undigested samples from sample 3 to confirm its DNA content before we proceed. If sample 3 shows that it is liable to digestion, we will proceed with PCR. If not, we will return to our other samples and may also extract DNA from ''Serratia marcescens'' with a new extraction protocol.	+	* On Tuesday, we ran gel electrophoresis on our now concentrated genomic DNA samples. Only sample 3 (from samples 1, 2, 3, and 5) produced a visible band. We did not feel confident that this was DNA, so today we ran digested and undigested samples from sample 3 to confirm its DNA content before we proceed. If sample 3 shows that it is liable to digestion, we will proceed with PCR. If not, we will return to our other samples and may also extract DNA from ''Serratia marcescens'' with a new extraction protocol.

Stephanie Vondrak at 21:19, 11 October 2012

Stephanie Vondrak — Thu, 11 Oct 2012 21:19:03 GMT

←Older revision		Revision as of 21:19, 11 October 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
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-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Prodigiosin Production in E.Coli</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Confirmation of DNA content==
-	* ~~Insert~~ content ~~here~~...	+	* On Tuesday, we ran gel electrophoresis on our now concentrated DNA samples. Only sample 3 (from samples 1, 2, 3, and 5) showed significant protein content. We did not feel confident that this protein product was DNA, so today we ran digested and undigested samples from sample 3 to confirm its DNA content before we proceed. If sample 3 shows that it is liable to digestion, we will proceed with PCR. If not, we will return to our other samples and may also extract DNA from ''Serratia marcescens'' with a new extraction protocol.

Stephanie Vondrak: Autocreate 2012/10/11 Entry for 840:153g:Projects/project22

Stephanie Vondrak — Thu, 11 Oct 2012 21:03:40 GMT

Autocreate 2012/10/11 Entry for 840:153g:Projects/project22

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

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[[category:OWWLabNotebookV1]]