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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Prodigiosin Production in E.Coli</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==PCR and Gel Electrophoresis Check== | ||
On Tuesday we decided that even though the gel electrophoresis was flawed (see picture - to be added) we felt we had sufficient DNA to proceed to PCR. Our purpose was two-fold - to make sure the positive control worked (last time we ran PCR it did not) and to see if our DNA was truly present in the sample (PCR would not amplify the gene if DNA wasn't there). | |||
On Thursday, we ran gel electrophoresis to check our PCR (insert picture). As you can see, our positive control worked, but our samples did not. It is important to note that our annealing time was only 1 minute 30 seconds and our target gene is 1470 b.p., so that may have an effect on our outcome. It is also possible our DNA is compromised and we will need to perform another DNA extraction. | |||
For next Tuesday, we will increase the annealing time for PCR and see if we get better results. We will also grow up more cultures of ''S. marcescens'' and prepare for DNA extraction. | |||
Revision as of 14:54, 18 October 2012
 Prodigiosin Production in E.Coli
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
PCR and Gel Electrophoresis CheckOn Tuesday we decided that even though the gel electrophoresis was flawed (see picture - to be added) we felt we had sufficient DNA to proceed to PCR. Our purpose was two-fold - to make sure the positive control worked (last time we ran PCR it did not) and to see if our DNA was truly present in the sample (PCR would not amplify the gene if DNA wasn't there). On Thursday, we ran gel electrophoresis to check our PCR (insert picture). As you can see, our positive control worked, but our samples did not. It is important to note that our annealing time was only 1 minute 30 seconds and our target gene is 1470 b.p., so that may have an effect on our outcome. It is also possible our DNA is compromised and we will need to perform another DNA extraction. For next Tuesday, we will increase the annealing time for PCR and see if we get better results. We will also grow up more cultures of S. marcescens and prepare for DNA extraction.
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