840:153g:Projects/project22/2012/10/25: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Prodigiosin Production in E.Coli</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Prodigiosin Production in E.Coli</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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[[Image:25.10.12_small.jpg]] Lane 3 marker on the right to Lane 12 marker on the left | [[Image:25.10.12_small.jpg]] Lane 3 marker on the right to Lane 12 marker on the left | ||
As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results (gel electrophoresis checks with questionable DNA content - I.E. indistinguishable from random proteins) with this DNA since day 1, so we will start preparing for another DNA extraction next week. | As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results (gel electrophoresis checks with questionable DNA content - I.E. results indistinguishable from random proteins) with this DNA since day 1, so we will start preparing for another DNA extraction next week. | ||
* We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol. | * We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol. |
Latest revision as of 22:09, 26 September 2017
Prodigiosin Production in E.Coli | Main project page Previous entry Next entry |
Attempted PCR and Gel Electrophoresis Verification
Lane 3 marker on the right to Lane 12 marker on the left As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results (gel electrophoresis checks with questionable DNA content - I.E. results indistinguishable from random proteins) with this DNA since day 1, so we will start preparing for another DNA extraction next week.
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