840:153g:Projects/project22/2012/10/25 - Revision history

Brian Hovey: /* Attempted PCR and Gel Electrophoresis Verification */

2012-10-25T21:34:13Z

Attempted PCR and Gel Electrophoresis Verification

←Older revision		Revision as of 21:34, 25 October 2012
Line 13:		Line 13:
	[[Image:25.10.12_small.jpg‎]] Lane 3 marker on the right to Lane 12 marker on the left		[[Image:25.10.12_small.jpg‎]] Lane 3 marker on the right to Lane 12 marker on the left

-	As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results (gel electrophoresis checks with questionable DNA content - I.E. indistinguishable from random proteins) with this DNA since day 1, so we will start preparing for another DNA extraction next week.	+	As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results (gel electrophoresis checks with questionable DNA content - I.E. results indistinguishable from random proteins) with this DNA since day 1, so we will start preparing for another DNA extraction next week.

	* We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol.		* We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol.

Brian Hovey: /* Attempted PCR and Gel Electrophoresis Verification */

2012-10-25T21:33:47Z

Attempted PCR and Gel Electrophoresis Verification

←Older revision		Revision as of 21:33, 25 October 2012
Line 13:		Line 13:
	[[Image:25.10.12_small.jpg‎]] Lane 3 marker on the right to Lane 12 marker on the left		[[Image:25.10.12_small.jpg‎]] Lane 3 marker on the right to Lane 12 marker on the left

-	As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results with this DNA since day 1, so we will start preparing for another DNA extraction next week.	+	As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results (gel electrophoresis checks with questionable DNA content - I.E. indistinguishable from random proteins) with this DNA since day 1, so we will start preparing for another DNA extraction next week.

	* We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol.		* We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol.

Brian Hovey: /* Attempted PCR and Gel Electrophoresis Verification */

2012-10-25T21:31:33Z

Attempted PCR and Gel Electrophoresis Verification

←Older revision		Revision as of 21:31, 25 October 2012
Line 11:		Line 11:
	* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.		* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.

-	[[Image:25.10.12_small.jpg‎]]	+	[[Image:25.10.12_small.jpg‎]] Lane 3 marker on the right to Lane 12 marker on the left

	As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results with this DNA since day 1, so we will start preparing for another DNA extraction next week.		As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results with this DNA since day 1, so we will start preparing for another DNA extraction next week.

Brian Hovey: /* Attempted PCR and Gel Electrophoresis Verification */

2012-10-25T21:30:14Z

Attempted PCR and Gel Electrophoresis Verification

←Older revision		Revision as of 21:30, 25 October 2012
Line 10:		Line 10:
	==Attempted PCR and Gel Electrophoresis Verification==		==Attempted PCR and Gel Electrophoresis Verification==
	* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.		* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.
		+
		+	[[Image:25.10.12_small.jpg‎]]
		+
		+	As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results with this DNA since day 1, so we will start preparing for another DNA extraction next week.

	* We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol.		* We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol.

Brian Hovey: /* Attempted PCR and Gel Electrophoresis Verification */

2012-10-25T20:43:29Z

Attempted PCR and Gel Electrophoresis Verification

←Older revision		Revision as of 20:43, 25 October 2012
Line 10:		Line 10:
	==Attempted PCR and Gel Electrophoresis Verification==		==Attempted PCR and Gel Electrophoresis Verification==
	* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.		* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.
-	We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol.	+
		+	* We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol.

Brian Hovey at 20:42, 25 October 2012

2012-10-25T20:42:45Z

←Older revision		Revision as of 20:42, 25 October 2012
Line 1:		Line 1:
	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Prodigiosin Production in E.Coli</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-

Stephanie Vondrak: /* Prodigiosin Production in E. Coli */

2012-10-25T20:39:00Z

Prodigiosin Production in E. Coli

←Older revision		Revision as of 20:39, 25 October 2012
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	== ~~Prodigiosin Production in E. Coli~~ ==	+	== ==
		+
	==Attempted PCR and Gel Electrophoresis Verification==		==Attempted PCR and Gel Electrophoresis Verification==
	* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.		* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.

Stephanie Vondrak: /* Attempted PCR and Gel Electrophoresis Verification */

2012-10-25T20:38:38Z

Attempted PCR and Gel Electrophoresis Verification

←Older revision		Revision as of 20:38, 25 October 2012
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
		+	== Prodigiosin Production in E. Coli ==
	==Attempted PCR and Gel Electrophoresis Verification==		==Attempted PCR and Gel Electrophoresis Verification==
	* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.		* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.

Stephanie Vondrak: /* Entry title */

2012-10-25T20:34:09Z

Entry title

←Older revision		Revision as of 20:34, 25 October 2012
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Attempted PCR and Gel Electrophoresis Verification==
-	* ~~Insert content here~~...	+	* In previous PCR runs, we did not obtain visible bands. We suspect that this was because the annealing time was not long enough. On Tuesday, we began another run of PCR with a 3 minute long annealing time. It was later discovered that the PCR cycle order was incorrect, so we then attempted to run the PCR again with the correct cycle orders. Today, we ran a gel on our PCR products to see if the PCR worked or not.
		+	We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol.

Stephanie Vondrak: Autocreate 2012/10/25 Entry for 840:153g:Projects/project22

2012-10-25T20:25:30Z

Autocreate 2012/10/25 Entry for 840:153g:Projects/project22

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__
[[category:OWWLabNotebookV1]]