840:153g:Projects/project22/2012/11/08: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Brian Hovey (talk | contribs) |
(fix raw html notebook nav) |
||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Prodigiosin Production by E. Coli</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Prodigiosin Production by E. Coli</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Gel Electrophoresis and PCR Setup== | ==Gel Electrophoresis and PCR Setup== | ||
* Upon our second extraction, we have been able to isolate DNA. We know this because on Tuesday we ran gel electrophoresis and had very bright bands that correspond with DNA. Today, we digested our samples and ran them through another gel to determine if they were digestible (using EcoR1). We did see a size difference between digested and undigested samples (lanes 13,15 and 17 were undigested and lanes 12, 14 and 16 were digested), except for in sample 3, which was in lanes 12 and 13. Lane 12 did not produce a band at all, and lane 13 produced an abnormal band ('streakier' than our other digested samples. | * Upon our second extraction, we have been able to isolate DNA. We know this because on Tuesday we ran gel electrophoresis and had very bright bands that correspond with DNA. Today, we digested our samples and ran them through another gel to determine if they were digestible (using EcoR1). We did see a size difference between digested and undigested samples (lanes 13,15 and 17 were undigested and lanes 12, 14 and 16 were digested), except for in sample 3, which was in lanes 12 and 13. Lane 12 did not produce a band at all, and lane 13 produced an abnormal band ('streakier' than our other digested samples). | ||
[[Image:Small_gel_11.08.12.png]] <-- Note the differences in lanes 12 and 13 from lanes 14-17 | [[Image:Small_gel_11.08.12.png]] <-- Note the differences in lanes 12 and 13 from lanes 14-17 |
Latest revision as of 22:13, 26 September 2017
Prodigiosin Production by E. Coli | Main project page Previous entry Next entry |
Gel Electrophoresis and PCR Setup
<-- Note the differences in lanes 12 and 13 from lanes 14-17
|