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PCR DNA Gel Extraction and Purification and the Beginning of T Vector Ligation
On Tuesday we ran a gel electrophoresis check on our PCR and it yielded these results:
Based on these results, we believe we have amplified our target gene (pigI) so we extracted the DNA from the gel and purified it with a GeneJet Gel Extraction kit (Lot 00040498). We ran a gel electrophoresis check on the sample obtained from gel extraction and it yielded these results:
This gel confirms our belief that we have our target gene, so we proceeded to the next step which is transformation and cloning of our DNA using a T-vector. To this end, we started the T Vector Ligation, using the Promega pGEM-T Easy Vector Ligation "Cloning PCR" protocol and will begin transformation on next Tuesday.