840:153g:Projects/project23/2012/09/27

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(Autocreate 2012/09/27 Entry for 840:153g:Projects/project23)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of the OmcF gene</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Entry title==
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* Insert content here...
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* We began the Transformation process of adding our vector, psB2k3 to E.coli
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* We successfully plated our transformed E. coli onto three different types of media and let incubate and then refrigerate
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* On Thursday, we received our bacteria and began working on the DNA extraction
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* We were able to make it through the first have of the extraction process, adding our buffer, spinning it down, removing the supernatant, adding TE, SDS, and EDTA, incubated it, added isopropanol and let store until the next class period

Revision as of 16:05, 27 September 2012

Cloning of the OmcF gene Main project page
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Entry title

  • We began the Transformation process of adding our vector, psB2k3 to E.coli
  • We successfully plated our transformed E. coli onto three different types of media and let incubate and then refrigerate
  • On Thursday, we received our bacteria and began working on the DNA extraction
  • We were able to make it through the first have of the extraction process, adding our buffer, spinning it down, removing the supernatant, adding TE, SDS, and EDTA, incubated it, added isopropanol and let store until the next class period


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