840:153g:Projects/project23/2012/09/27

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Cloning of the OmcF gene Main project page
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  • We began the Transformation process of adding our vector, psB2k3 to E.coli
  • We successfully plated our transformed E. coli onto three different types of media and let incubate and then refrigerate
  • On Thursday, we received our bacteria and began working on the DNA extraction
  • We were able to make it through the first have of the extraction process, adding our buffer, spinning it down, removing the supernatant, adding TE, SDS, and EDTA, incubated it, added isopropanol and let store until the next class period


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