840:153g:Projects/project23/2012/10/11

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of the OmcF gene</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Cloning of the OmcF gene==
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* On Tuesdays, we grew the E.coli that we had received from the Parts Registry; having ordered it the previous Tuesday. This part was vector psB2K3 which contained the promoter BBa_I0500. We plated the bacteria on KanR LB media.
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* On Wednesday we transferred the bacterial cultures into liquid LB media to increase the amount of bacteria to use for extracting the plasmid out again.
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* On Thursday, we used the GeneJet Plasmid Miniprep Kit to extract our plasmid DNA from the E.coli that we grew on Tuesday and Wednesday
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* We ran an agrose to gel to verify the existence of plasmid DNA. We used 5 wells in the gel. In wells 1-4 we placed 5 microL of DNA, with our samples, A-D. In well 5 we placed 2 microL of 100 bp ladder.
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Our Results:
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Revision as of 14:19, 16 October 2012

Cloning of the OmcF gene Main project page
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Cloning of the OmcF gene

  • On Tuesdays, we grew the E.coli that we had received from the Parts Registry; having ordered it the previous Tuesday. This part was vector psB2K3 which contained the promoter BBa_I0500. We plated the bacteria on KanR LB media.
  • On Wednesday we transferred the bacterial cultures into liquid LB media to increase the amount of bacteria to use for extracting the plasmid out again.
  • On Thursday, we used the GeneJet Plasmid Miniprep Kit to extract our plasmid DNA from the E.coli that we grew on Tuesday and Wednesday
  • We ran an agrose to gel to verify the existence of plasmid DNA. We used 5 wells in the gel. In wells 1-4 we placed 5 microL of DNA, with our samples, A-D. In well 5 we placed 2 microL of 100 bp ladder.

Our Results:


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