840:153g:Projects/project23/2012/10/11: Difference between revisions

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* On Wednesday we transferred the bacterial cultures into liquid LB media to increase the amount of bacteria to use for extracting the plasmid out again.
* On Wednesday we transferred the bacterial cultures into liquid LB media to increase the amount of bacteria to use for extracting the plasmid out again.
* On Thursday, we used the GeneJet Plasmid Miniprep Kit to extract our plasmid DNA from the E.coli that we grew on Tuesday and Wednesday
* On Thursday, we used the GeneJet Plasmid Miniprep Kit to extract our plasmid DNA from the E.coli that we grew on Tuesday and Wednesday
* We ran an agrose to gel to verify the existence of plasmid DNA. We used 5 wells in the gel. In wells 1-4 we placed 5 microL of DNA, with our samples, A-D. In well 5 we placed 2 microL of 100 bp ladder.
* We ran an agrose to gel to verify the existence of plasmid DNA. We used 5 wells in the gel. In wells 1-4 we placed 5 mcL each of our purified plasmid DNA, samples A-D respectively. In well 5 we placed 2 mcL of 100 bp ladder.


Our Results:
Our Results: Each prepared plasmid DNA sample showed faint but usable quality DNA.


[[Image:E coli plasmid DNA.jpg]]


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[[category:OWWLabNotebookV1]]
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Revision as of 18:08, 16 October 2012

Cloning of the OmcF gene <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Cloning of the OmcF gene

  • On Tuesdays, we grew the E.coli that we had received from the Parts Registry; having ordered it the previous Tuesday. This part was vector psB2K3 which contained the promoter BBa_I0500. We plated the bacteria on KanR LB media.
  • On Wednesday we transferred the bacterial cultures into liquid LB media to increase the amount of bacteria to use for extracting the plasmid out again.
  • On Thursday, we used the GeneJet Plasmid Miniprep Kit to extract our plasmid DNA from the E.coli that we grew on Tuesday and Wednesday
  • We ran an agrose to gel to verify the existence of plasmid DNA. We used 5 wells in the gel. In wells 1-4 we placed 5 mcL each of our purified plasmid DNA, samples A-D respectively. In well 5 we placed 2 mcL of 100 bp ladder.

Our Results: Each prepared plasmid DNA sample showed faint but usable quality DNA.

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