840:153g:Projects/project23/2012/10/25

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(Continuation of the Mutagenesis Reaction)
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==Continuation of the Mutagenesis Reaction ==
==Continuation of the Mutagenesis Reaction ==
-
*  
+
 
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* On Tuesday, we ran the gel for last week's PCR products.  This was done on a dual-lane chamber, loaded as follows:
 +
 
 +
 
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*Top Lane:
 +
#100 bp ladder
 +
#P+55
 +
#P+60
 +
#P+65
 +
#Positive Control
 +
#P-55
 +
#P-60
 +
#P-65
 +
#100 bp ladder
 +
 
 +
 
 +
*Bottom Lane:
 +
#100 bp ladder
 +
#N+55
 +
#N+60
 +
#N+65
 +
#Positive Control
 +
#N-55
 +
#N-60
 +
#N-65
 +
#100 bp ladder
 +
 
 +
 
 +
*We ran these on a 1% agarose gel for 30min at 120V
 +
 
 +
 
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*Results:
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 +
 

Revision as of 17:54, 29 October 2012

Cloning of the OmcF gene Main project page
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Continuation of the Mutagenesis Reaction

  • On Tuesday, we ran the gel for last week's PCR products. This was done on a dual-lane chamber, loaded as follows:


  • Top Lane:
  1. 100 bp ladder
  2. P+55
  3. P+60
  4. P+65
  5. Positive Control
  6. P-55
  7. P-60
  8. P-65
  9. 100 bp ladder


  • Bottom Lane:
  1. 100 bp ladder
  2. N+55
  3. N+60
  4. N+65
  5. Positive Control
  6. N-55
  7. N-60
  8. N-65
  9. 100 bp ladder


  • We ran these on a 1% agarose gel for 30min at 120V


  • Results:



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