840:153g:Projects/project23/2012/10/25: Difference between revisions
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**Template dilution works well for either high or low concentrations. We will use 1:200 for further amplification. | **Template dilution works well for either high or low concentrations. We will use 1:200 for further amplification. | ||
**Annealing temperature did not seem to vary results for this range. | **Annealing temperature did not seem to vary results for this range. | ||
*After analyzing this gel, we decided to run another PCR to do a side-by-side comparison of the two fragments and the combined reaction, to see if they are separate products. We used only the 1:200 template, and the 65°C annealing temperature, as they gave us the cleanest images to date. We prepared an upstream and downstream reaction from our original total-DNA sample, and primered/ non-primered set identical to earlier this week. We also prepared each of these sets without template DNA for negative control. We cycled our PCR with the same protocol we used in the past, but with a non-gradient annealing temperature. | |||
Revision as of 17:20, 29 October 2012
Cloning of the OmcF gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Continuation of the Mutagenesis Reaction
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