840:153g:Projects/project23/2012/10/25: Difference between revisions
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==Continuation of the Mutagenesis Reaction == | ==Continuation of the Mutagenesis Reaction == | ||
* | |||
* On Tuesday, we ran the gel for last week's PCR products. This was done on a dual-lane chamber, loaded as follows: | |||
*Top Lane: | |||
#100 bp ladder | |||
#P+55 | |||
#P+60 | |||
#P+65 | |||
#Positive Control | |||
#P-55 | |||
#P-60 | |||
#P-65 | |||
#100 bp ladder | |||
*Bottom Lane: | |||
#100 bp ladder | |||
#N+55 | |||
#N+60 | |||
#N+65 | |||
#Positive Control | |||
#N-55 | |||
#N-60 | |||
#N-65 | |||
#100 bp ladder | |||
*We ran these on a 1% agarose gel for 30min at 120V | |||
*Results: | |||
Revision as of 14:54, 29 October 2012
Cloning of the OmcF gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Continuation of the Mutagenesis Reaction
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