840:153g:Projects/project23/2012/10/26: Difference between revisions

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==Entry title==
==Site-directed Mutagenesis==
* Insert content here...
* On Tuesday, we ran the gel for last week's PCR products.   This was done on a dual-lane chamber, loaded as follows:
 
 
*Top Lane:
#100 bp ladder
#P+55
#P+60
#P+65
#Positive Control
#P-55
#P-60
#P-65
#100 bp ladder
 
 
*Bottom Lane:
#100 bp ladder
#N+55
#N+60
#N+65
#Positive Control
#N-55
#N-60
#N-65
#100 bp ladder
 
 
*We ran these on a 1% agarose gel for 30min at 120V
 
 
*Results:
 




Revision as of 14:50, 29 October 2012

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Site-directed Mutagenesis

  • On Tuesday, we ran the gel for last week's PCR products. This was done on a dual-lane chamber, loaded as follows:


  • Top Lane:
  1. 100 bp ladder
  2. P+55
  3. P+60
  4. P+65
  5. Positive Control
  6. P-55
  7. P-60
  8. P-65
  9. 100 bp ladder


  • Bottom Lane:
  1. 100 bp ladder
  2. N+55
  3. N+60
  4. N+65
  5. Positive Control
  6. N-55
  7. N-60
  8. N-65
  9. 100 bp ladder


  • We ran these on a 1% agarose gel for 30min at 120V


  • Results:


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